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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ, IL-10 and Granzyme B
Analyte Information
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-10/Granzyme B FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
Pre-coated plates enable a more rapid assay with minimized variability.
Analyte Information
Granzyme BGranzyme B (also known as GzB or GzmB) is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act in concert with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
IL-10Interleukin-10 (IL-10, IL10) is a pleiotropic cytokine that is produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Format: | FluoroSpotPLUS |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
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| Size: | 10 plates |
| Other formats: | Click to view all Human Granzyme B, IFN-γ, IL-10 products |
We have used Mabtech ELISPOT antibodies and reagents for several years. The high quality and standard and competitive price will keep us hooked for years to come. Recently we have started working with Fluorospot, which has really helped us move forward and have opened new avenues in our research. As always management and technical staff have all provided impeccable service. | |
