Specifications&Handling:

ProductData
Synonyms	PA700
Properties
Source/Host	HEK293cells
Sequence	Human19SProteasome-UCHL5(C88A)fusedtoaN-terminalFLAG-tag.
Crossreactivity	Human
Formulation	Liquid.InHEPES,NaCl,GlycerolandDTT.
OtherProductData	Use:Multi-subunitregulatorycomponentofthe26Sproteasome.Reactionconditionswillneedtobeoptimizedforeachspecificapplication.Werecommendaninitialproteinconcentrationof0.5-5nM.This19SProteasomepreparationwaspurifiedwithaninactiveUCHL5enzyme(Cys88toAlamutation)andmaybeusedfordeubiquitinationoftargetsubstratesviaPOH1orUSP14.
Origin	ManufacturedbyBostonBiochem
ProductType	Protein
ShippingandHandling
Shipping	DRYICE
ShortTermStorage	-20°C
LongTermStorage	-80°C
HandlingAdvice	Aliquottoavoidfreeze/thawcycles.
Use/StABIlity	Stableforatleast1yearafterreceiptwhenstoredat-80°C.

Description:

The19SProteasome,alsoknownasPA700,isamulti-subunitregulatoryparticlethatassociateswiththe20SProteasomecoreparticletoformthe26SProteasome.The19SProteasomecancapthe20SProteasomeatoneorbothendsandregulatesubstrateaccesstoproteolyticactivityinanATP-dependentmannerbymodulatingtheconformationofthe20SProteasomeandfacilitatingsubstrateaccesstothe20SProteasomecatalyticcore.The19SProteasomerecognizesubiquitinatedproteinsaswellasnon-ubiquitinated,misfoldedproteins.Itcatalyzessubstratedeubiquination,unfoldssubstrateproteins,andthreadsthemintothe20SProteasomecatalyticcore.The19SProteasomeconsistsofabasesubcomplexandalidsubcomplex.ThebasesubcomplexiscomposedofsixAAA+familymembers,twoscaffoldingproteins,andregulatoryproteinsinvolvedinubiquitinrecognition.The19SProteasomelidsubcomplexcontainseightsubunitsandonedeubiquitinatingenzyme,POH1/Rpn11,presentatstoichiometriclevels.Twootherdeubiquitinatingenzymes,UCHL5(alsoknownasUch37)andUSP14arereversIBLyassociatedwiththe19SProteasome. AdipoGen生命科学有限公司（AdipoGen）由两家公司组成，分别是Adipogen SA（瑞士）（及其分支机构Adipogen AG）和Adipogen Corporation（美国圣地亚哥）。公司总部位于美国圣地亚哥，而运营总部位于瑞士利斯塔尔。一支由高技能人士组成的积极团队，为肥胖和糖尿病，炎性疾病，先天免疫，免疫学，免疫肿瘤学，癌症，干细胞生物学和神经变性等领域的生命科学研究市场开发和制造新产品，以支持免疫代谢和高级炎症研究。除了抗体（特殊功能抗体）和蛋白质设施外，AdipoGen还拥有化学实验室，使该公司能够分离出独特的天然产物，并开发出创新的小分子和稀有抗生素。自2015年以来，AdipoGen在国际上以“ AdipoGen Life Sciences”品牌运营。除研究试剂外，AdipoGen还正在开发新产品，以在新的市场领域进行许可（临床诊断/治疗）。