Tested and Certified for in vivo imaging (See "Luciferin FAQ" in additional information)

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C.Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging.Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation.The injection of Luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

L-Luciferin is the chiralic sister to D-luciferin.While most researchers claim that L-luciferin does not produce any light, Lembert has reported that it produces a very weak and extremely slow manifesting light (Lembert, 1996).Nakamura suggested that an enzyme system incorporating luciferase might be responsible for changing the chirality of the L-luciferin to D-luciferin (Nakamura et al., 2006) (See Reference Library).

Product Specifications:L-Luciferin, Potassium Salt4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt

Forms a yellow, clear solution when dissolved 1% in water.

Formula: C₁₁H₇N₂O₃S₂K

MW: 318.42 g/mol

Color: Light yellow powder

Purity: >98% by HPLC

Storage/Handling: Store desiccated in -20°C, and protect from light.

PubChem CID: 135845006

小鼠 1.       首先确定针的入口点。 2.       在膝关节的上部横跨腹部画一条想像的线。 3.       针应该沿着动物右侧这条线并贴近中间插入。（对于雌鼠，针的入口点位于最后一个乳头稍靠中间的部位。） 4.       针从小鼠的右侧插入避开盲肠，盲肠是腹部左侧一个大的充满液体的器官。小肠（位于右侧）不大可能被针刺穿。 5.       针的插入从插入点离尾部或横向太远如上显示将是危险的，可能会注射到后腿里，这样将会造成肌肉组织受伤。 6.       为完成腹腔注射，必须很好地控制好小鼠以防在操作过程中移动。一旦针插入到腹部避免器官受损伤。 7.       控制好小鼠并倾斜以便使其头向下，腹部露出来。 8.       用70%乙醇对注射位点进行彻底消毒。 9.       对于腹部注射我们建议用25-27 gauge直径的针，以30度角度插入腹部。 10.   针应插入大约半厘米（4-5mm）的深度。 11.   轻轻地吸出以确认针没有穿透血管，肠或膀胱。    a.       褐绿色吸出物表示针插进了小肠。    b.       黄色吸出物暗示针插进了膀胱。 12.   如果任何液体被吸出，表示您的溶液被污染了必须弃掉并用一个新的注射器和针头重新操作。 13.   如果没有液体被吸出，你可以开始注射。 14.   取出针头并记录下注射的时间，以确定信号达峰时间。 大鼠 按照小鼠腹腔注射操作流程。对于大鼠我们建议用一个25 gauge 直径的针头注射。 注意 l  确保针是倾斜的。 l  使动物的头倾斜向下为了使肠内物向下移动，以保持腹部四分区下部为空腔。 l  为了精确注射深度防止深层注射，我们建议放一根导管在针头上留出4-5mm为合适的深度。 l  确保注射体积，我们建议小鼠不超过2ml，大鼠不超过5ml（请参看附录关于不同动物和位点的不同注射体积。） l  给大鼠注射时，用布盖在大鼠的头部防止小鼠和研究人员紧张。 静脉注射