GoldBio/MES Free Acid Monohydrate, Ultra Pure/100 g/M-090-100

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货号:M-090-100
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品牌:GoldBio
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商品描述

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.

Product SpecificationsMES Free Acid, Monohydrate, Ultra Pure2-(N-Morpholino)ethanesulfonic acid, Monohydrate

Formula: C6H13NO4S ∙ H2O

MW: 213.25 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 16218417

Note: MES Hydrate (CAS# 1266615-59-1) and MES Monohydrate (CAS# 145224-94-8) differ only in the number of water molecules associated with the buffer in powder form. Regardless of the number of water molecules present, these buffers are identical when dissolved into an aqueous solution.

小鼠 1.       首先确定针的入口点。 2.       在膝关节的上部横跨腹部画一条想像的线。 3.       针应该沿着动物右侧这条线并贴近中间插入。(对于雌鼠,针的入口点位于最后一个乳头稍靠中间的部位。) 4.       针从小鼠的右侧插入避开盲肠,盲肠是腹部左侧一个大的充满液体的器官。小肠(位于右侧)不大可能被针刺穿。 5.       针的插入从插入点离尾部或横向太远如上显示将是危险的,可能会注射到后腿里,这样将会造成肌肉组织受伤。 6.       为完成腹腔注射,必须很好地控制好小鼠以防在操作过程中移动。一旦针插入到腹部避免器官受损伤。 7.       控制好小鼠并倾斜以便使其头向下,腹部露出来。 8.       用70%乙醇对注射位点进行彻底消毒。 9.       对于腹部注射我们建议用25-27 gauge直径的针,以30度角度插入腹部。 10.   针应插入大约半厘米(4-5mm)的深度。 11.   轻轻地吸出以确认针没有穿透血管,肠或膀胱。    a.       褐绿色吸出物表示针插进了小肠。    b.       黄色吸出物暗示针插进了膀胱。 12.   如果任何液体被吸出,表示您的溶液被污染了必须弃掉并用一个新的注射器和针头重新操作。 13.   如果没有液体被吸出,你可以开始注射。 14.   取出针头并记录下注射的时间,以确定信号达峰时间。 大鼠 按照小鼠腹腔注射操作流程。对于大鼠我们建议用一个25 gauge 直径的针头注射。 注意 l  确保针是倾斜的。 l  使动物的头倾斜向下为了使肠内物向下移动,以保持腹部四分区下部为空腔。 l  为了精确注射深度防止深层注射,我们建议放一根导管在针头上留出4-5mm为合适的深度。 l  确保注射体积,我们建议小鼠不超过2ml,大鼠不超过5ml(请参看附录关于不同动物和位点的不同注射体积。) l  给大鼠注射时,用布盖在大鼠的头部防止小鼠和研究人员紧张。 静脉注射