GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Glial Cell-derived Neurotrophic FactorGDNFATF1, ATF2Astrocyte-derived trophic factor

Glial cell-derived neurotrophic factor (GDNF) is a small protein that promotes the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. GDNF is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cystine knot configuration.

Formulation: Sterile filtered whitelyophilized (freeze-dried) powder.

Sequence: SPDKQMAVLPRRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYDKILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI

MW: ~30.1 kDa (a homodimeric protein consisting of two 134 aminoacid non-glycosylated polypeptide chains)

Purity: >97% by SDS-PAGEand HPLC analyses

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of >5.0 × 10⁷ IU/mg.

Accession Number: P39905

HGNC ID: 4232

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long term storage.The reconstituted sample can be apportioned into working aliquots and storedat -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutions shouldbe made in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!

Note: 1000 µg volume may be delivered in multiple containers.

小鼠 1.       首先确定针的入口点。 2.       在膝关节的上部横跨腹部画一条想像的线。 3.       针应该沿着动物右侧这条线并贴近中间插入。（对于雌鼠，针的入口点位于最后一个乳头稍靠中间的部位。） 4.       针从小鼠的右侧插入避开盲肠，盲肠是腹部左侧一个大的充满液体的器官。小肠（位于右侧）不大可能被针刺穿。 5.       针的插入从插入点离尾部或横向太远如上显示将是危险的，可能会注射到后腿里，这样将会造成肌肉组织受伤。 6.       为完成腹腔注射，必须很好地控制好小鼠以防在操作过程中移动。一旦针插入到腹部避免器官受损伤。 7.       控制好小鼠并倾斜以便使其头向下，腹部露出来。 8.       用70%乙醇对注射位点进行彻底消毒。 9.       对于腹部注射我们建议用25-27 gauge直径的针，以30度角度插入腹部。 10.   针应插入大约半厘米（4-5mm）的深度。 11.   轻轻地吸出以确认针没有穿透血管，肠或膀胱。    a.       褐绿色吸出物表示针插进了小肠。    b.       黄色吸出物暗示针插进了膀胱。 12.   如果任何液体被吸出，表示您的溶液被污染了必须弃掉并用一个新的注射器和针头重新操作。 13.   如果没有液体被吸出，你可以开始注射。 14.   取出针头并记录下注射的时间，以确定信号达峰时间。 大鼠 按照小鼠腹腔注射操作流程。对于大鼠我们建议用一个25 gauge 直径的针头注射。 注意 l  确保针是倾斜的。 l  使动物的头倾斜向下为了使肠内物向下移动，以保持腹部四分区下部为空腔。 l  为了精确注射深度防止深层注射，我们建议放一根导管在针头上留出4-5mm为合适的深度。 l  确保注射体积，我们建议小鼠不超过2ml，大鼠不超过5ml（请参看附录关于不同动物和位点的不同注射体积。） l  给大鼠注射时，用布盖在大鼠的头部防止小鼠和研究人员紧张。 静脉注射