SkeletalMuscleCellGrowthMedium

Skeletalmuscleisanimportantsiteofinsulin-stimulatedglucosedisposalandoftenthesiteofinsulinresistanceinobesity.Humanprimaryculturedskeletalmyoblastscandirectlyreflectapatient"smetabolicphenotype,becausemanyofthesignalingpathwaysaremaintainedintact.Zenbiooffershumanprimaryskeletalmyoblastsfromavarietyofdonors,includingobesedonorswithType2diabetes.

HumanskeletalmyoblastssatellitecellsareisolatedfromconsentedpatientswithgoodrepresentationfromalllevelsofADIposityandage.Skeletalmyoblastsareavailablefrombothmaleandfemaledonors.PooledlotsofcellsareavailabletoprovidelargelotsforscreeningandtodecreaseissueswithpatienttopatientvariABIlity.

Cellsareavailablecryopreserved(500,000cells/vial).

QUALITYCONTROL:Allcellshavebeentestedformyotubedifferentiation.CellshavetestednegativeforHIV-1,HIV-2,HTLC-1,HTLV-2,HepatitisB,HepatitisCandmycoplasma.HumanskeletalmyoblastaredifferentiatedusingSkeletalMuscleDifferentiationMedium(cat#SKM-D)foratleast6days.Myogenicdifferentiationwasverifiedbyimmunohistochemicalstainingusingantibodiestodesminandmyosin.Thedifferentiatedmyocytesalsorespondtoinsulinstimulatedglucoseuptake.

Speciallyformulatedmediaareavailableformaintenanceanddifferentiationofouradultskeletalmyoblasts.

ZenBio提供了多种检测方法来检测作为潜在糖尿病或肥胖疗法的化合物。客户可以从以下任何一种检测方法中进行选择，或者我们可以设计一个针对每个客户的特定研究需求的定制程序。