Papain,LyophilizedSuppliedasalyophilizedpowderpreparedfromatwotimescrystallinesUSPension,Code:PAP.Toinsurefullactivity,theenzymeshouldbeincubatedinasolutioncontaining1.1mMEDTA,0.067mMmercaptoethanoland5.5mMcysteine-HClfor30minutes.Itisrecommendedthattheenzymebe0.22micronfilteredafterdissolutionandpriortouse.Storeat2-8°C.Activatestoatleast15unitspermgproteinSource:CaricaPapayaLatex

I.U.B.:3.4.22.2

PapainisasulfhydrylproteasefromCaricapapayalatex.Ithasamolecularweightof23kDaandanoptimumpHrangeof6.0-7.0. Theactionofpapainonleucinemethylesterproducesaninsolublepolyleucinepeptide.Papainbreaksdowntheintercellularmatrixofcartilage.Papainisactivatedbycysteine,sulfide,andsulfite.StABIlizingagentsareEDTA,cysteineanddimercaptoethanol.

Stability/Storage:Stablefor6-12monthsat2-8°C. Donotfreezeaqueoussuspensions.

TechnicalNotes:Papainpreparationsshouldbeincubatedintheactivationsolutionbeforeusetoensurefullactivity.Applicationsincludeantibodyfragmentationandprimary/neuralcellisolation.

UnitDefinition:OneUnithydrolyzesonemicromoleofbenzoyl-L-arginineethylesterperminuteat25°C,pH6.2,afteractivationinasolutioncontaining1.1mMEDTA,0.067mMmercaptoethanoland5.5mMcysteine-HClfor30minutes.

RelatedProducts:

Collagenase(CLS1-4/CLSPA)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Hyaluronidase(HSE/HSEP)
NeutralProtease(Dispase,NPRO)
ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)
TrypsinInhibitors(LBI/OI/SI/SIC)

CellIsolationOptimizimgSystem(CIT)
HepatocyteIsolationSystem(HIS)
NeonatalCardiomyocyteIsolationSystem(NCIS)
PapainDissociiationSystem(PDS/PDS2)

美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。