Lysozyme,Purified,SaltFreeTwotimescrystallized.Adialyzed,lyophilizedpowder.Storeat2-8°C.≥8,000unitspermgdryweightSource:EggWhites

I.U.B.:3.2.1.17

Lysozymepreferentiallyhydrolyzestheβ-1,4glycosidiclinkagesbetweenN-acetylmuramicacidandN-acetylglucosaminewhichoccurinthemucopeptidecellwallstructureofcertainmicroorganisms,suchasMicrococcuslysodeikticus(Productcode:ML).Aslightlymorelimitedactivityisexhibitedtowardchitinoligomers.Ithasamolecularweightof14,388daltons.OptimumpHis9.2.Lysozymeisinhibitedbysurfaceactiveagentssuchasdodecylsulfate,alcoholandfattyacids.Imidazoleandindolederivativesforminhibitorychargedtransfercomplexes.

StABIlity/Storage:Stablefor3-5yearsat2-8°C.SolutionsatpH4-5arestableforseveralweeksrefrigeratedandfordaysatambienttemperatures.Storeat2-8°C.

TechnicalNote:Duetoassaydifferences,8,000u/mgbyWorthington"sassayisequivalentto50,000u/mgclaimedbyothersuppliers.

UnitDefinition:OneUnitisequaltoadecreaseinturbidityof0.001perminuteat450nmatpH7.0and25°C,usinga0.3mg/mlsUSPensionofMicrococcuslysodeikticuscells(WBCproductcodeML)assubstrate.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。