DeoxyribonucleicAcid,CalfThymusSource:CalfThymusHighlypolymerized;hyperchromicity27%.Asubstratefordeoxyribonucleaseassays.PreparedbyamethoddevelopedatWorthingtontoremovecontaminatingRNAandprotein.Supplieddried.Storeat2-8°C.N/A

WorthingtonoffersDNApurifiedfromthesesources:

Calfthymus:(Code:DNA)PreparedandpurifiedbyamethoddevelopedatWorthingtontohavelowerproteinandRNAcontaminationthanmostothercommercialpreparations.ThishighlypolymerizedDNAisanexcellentsubstratefordeoxyribonuclease.Asodiumsalt,itmustbeconvertedbyaddingmagnesiumionstobesusceptIBLetoDNase.

CalfthymusDNA,covalentlyboundtocelluloseisalsoavailable:
DNACellulose,Double-Stranded(Code:DNACELDS)
DNACellulose,Single-Stranded(Code:DNACELSS)

SalmonTestes:(Code:SDNA)PreparedbyamodificationofthemethodofEmanuelandChaikoff,J.Biol.Chem.,203,164,(1953).Aminimumof75%nativenucleicacid.

SalmonTestesDNA,Denatured&Fragmented(Code:SDNAD)isalsoavailable.Preparedbymechanicalshearingandheatdenaturation.

Escherichiacoli:(Code:DNAEC)IsolatedasdescribedbyMarmur,J.Mol.Biol.,3,208,1961.

LamBDaphageDNA(Code:DNAL)ispreparedfromCsClpurifiedphageandispurifiedtoanA₂₆₀/A₂₈₀≥1.8.Homogeneousinagarosegelelectrophoresis.Asolutionin10mMTris-HClpH8.0with1mMEDTA.

DNAfragmentspreparedbyrestrictionendonucleasedigestionofpurifiedlambdaDNAareavailable(Codes:DNALBSTE;DNALECOR;DNALHIND).Suppliedassolutionsin10mMTris-HClpH8.0with1mMEDTA.

TechnicalNote:OneA₂₆₀unit=50µgDNA.

StABIlity/Storage:DNAL:Storagebuffer10mMTris-HCl,pH8.0containing1mMEDTA.Storeat-20°C. Oncethawedkeepat2-8°C.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。