ReverseTranscriptase,Recombinant,HIVChromatographicallypurifieddimericformwithM.W.of66kDaand51kDa.Asolutionin10mMpotassiumphosphate,pH7.4,1mMDTTand20%glycerol.Storeat-20°C.REQUIRESSPECIALSHIPPING:DRYICE≥5,000unitspermgproteinSource:RecombinantproteinproducedinE.coli

I.U.B.:2.7.7.49

Chromatographicallypurifiedheterodimercomposedof66kDaand51kDasubunits.Suppliedasasolutionin10mMpotassiumphosphate,pH7.4,1mMDTTand20%glycerol.PrimarilyforAIDSresearchpurposes;thisenzymehaslessfidelitythantheAMVenzymeinotherapplicationssuchasthepreparationofCDNAfrommRNAforcloningpurposes.

UnitDefinition:OneUnitincorporates1nmoleoftritiatedd-TMPintoacidprecipitableproductsusingpoly(A)/oligo(dT)12-18asthetemplate/primerin20minutesat37°C,pH8.3.

Note:Thecurrentassayusesamoreefficienttemplate/primercombinationthatresultsinanapproximatetwo-foldincreaseinspecificactivitycomparedtoourpreviousassay.Asaresult,Worthington"spackagesizescontainthesameamountofproteinwithtwiceasmuchactivitybaseduponthenewassay.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。