StressMarq蚂蚁淘在线

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Peptide Affinity Purified
Clonality	Polyclonal
Specificity	Detects 84.902 kDa.
Cite This Product	StressMarq Biosciences Cat# SPC-1082, RRID: AB_2709875
Certificate of Analysis	A 1:250 dilution of SPC-1082 was sufficient for detection of SIK1 (pThr182) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	SNF1LK Antibody, KID2 Antibody, MSK Antibody, myocardial SNF1 like kinase Antibody, QIK Antibody, Qin-induced kinase Antibody, Salt inducible kinase 1 Antibody, Salt-inducible protein kinase 1 Antibody, Serine threonine protein kinase SNF1 like kinase 1 Antibody, Serine threonine protein kinase SNF1LK Antibody, Serine/threonine-protein kinase SIK1 Antibody, Serine/threonine-protein kinase SNF1-like kinase 1 Antibody, Serine/threonine-protein kinase SNF1LK Antibody, SIK-1 Antibody, SIK1 Antibody, SIK1_HUMAN Antibody, SNF1 like kinase Antibody
Cellular Localization	Cytoplasm, Nucleus
Accession Number	NP_775490
Gene ID	102724428
Swiss Prot	P57059
Scientific Background	SIK1 (salt-inducible kinase 1), also known as SNF1LK or MSK, is a 783 amino acid protein that contains one UBA domain and one protein kinase domain and belongs to the Ser/Thr protein kinase family. Localized to both the nucleus and the cytoplasm, SIK1 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation. Additionally, SIK1 has a potential role in regulation of the G2/M cell cycle transition, as well as in inhibitory control of CREB protein function.

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ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

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