StressMarq/Anti-JNK3 Antibody/SPC-1153D-APC/100-µl

价格
¥6820.00
货号:SPC-1153D-APC
浏览量:127
品牌:StressMarq
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Overview:

Product Name JNK3 Antibody
Description

Rabbit Anti-Human JNK3 Polyclonal

Species Reactivity Human
Applications WB, AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide of human JNK3 (AA305-319)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects ~53 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-1153, RRID: AB_2711153
Certificate of Analysis A 1:250 dilution of SPC-1153 was sufficient for detection of JNK3 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names c Jun kinase 3 antibody, c-Jun N-terminal kinase 3 antibody, cJun N terminal kinase 3 antibody, JNK3 alpha protein kinase antibody, JNK3 antibody, JNK3A antibody, MAP kinase 10 antibody, MAP kinase antibody, MAP kinase p49 3F12 antibody, MAPK 10 antibody, Mapk10 antibody, mitogen activated protein kinase 10 antibody, Mitogen-activated protein kinase 10 antibody, MK10_HUMAN antibody, PRKM10 antibody, protein kinase mitogen activated 10 antibody, SAPK1b antibody, Stress activated protein kinase 1b antibody
Cellular Localization Cytoplasm
Accession Number NP_620448
Gene ID 5602
Swiss Prot P53779
Scientific Background MAPK10 is a protein-serine/threonine kinase. The near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.

Product Images

Currently there are no images for this product

Product Citations (0)

Currently there are no citations for this product.

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

 APC Fluorophore Absorption and Emission SpectrumOptical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

StressMarq硫黄素T测定硫黄素T是一种荧光染料,可与富含β片层的结构(例如α突触核蛋白原纤维中的结构)结合。结合后,染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液(新鲜制备,并通过0.2μm注射器过滤器过滤)。在PBS pH 7.4中稀释硫黄素T,使每孔中硫黄素T的最终浓度为25μM(每孔体积= 100μL)。板:Lumox 96多孔板(Sarstedt目录号94.6000.024)。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体(或两者)添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值,需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱(600 rpm)中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置:温度:37°C读取类型:阱扫描波长:450 nm处的激发和485 nmPMT 处的发射增益:每次读取自动闪烁:6次摇动:读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。
  • 资质认证

    获得国家资质,权威认证!

  • 全国联保

    全国联保,官方无忧售后

  • 正规发票

    正规发票,放心购买

  • 签订合同

    签订合同,保障您的权益

/**/