StressMarq蚂蚁淘在线

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects endogenous levels of total ABCG2
Cite This Product	StressMarq Biosciences Cat# SPC-1317, RRID: AB_2713493
Certificate of Analysis	A 1:1000 dilution of SPC-1317 was sufficient for detection of ABCG2 in 10 µg of mouse kidney cell lyates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	ABC transporter antibody ABC15 antibody ABCG 2 antibody ABCG2 antibody ABCG2_HUMAN antibody ABCP antibody ATP binding cassette sub family G (WHITE) member 2 antibody ATP binding cassette transporter G2 antibody ATP-binding cassette sub-family G member 2 antibody BCRP antibody BCRP1 antibody BMDP antibody Breast cancer resistance protein antibody CD338 antibody CDw338 antibody CDw338 antigen antibody EST157481 antibody GOUT1 antibody MGC102821 antibody Mitoxantrone resistance associated protein antibody Mitoxantrone resistance-associated protein antibody MRX antibody Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody MXR antibody MXR1 antibody Placenta specific ATP binding cassette transporter antibody Placenta specific MDR protein antibody Placenta-specific ATP-binding cassette transporter antibody UAQTL1 antibody
Cellular Localization	Cell membrane, Mitochondrion membrane
Accession Number	NP_004818.2
Gene ID	9429
Swiss Prot	Q9UNQ0
Scientific Background	ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells. ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. Originally thought to play a role in drug resistance due to its resistance to a number of chemotherapeutic drugs, ABCG2 has also been found in the kidney, and stem cell populations. Studies also suggest that it can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells. The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface.
References	1. Zhou S., et al. (2001) Nat. Medicine. 7: 1028. 2. Doyle L.A. and Ross D.D. (2003) Oncogene. 22: 7340-58. 3. Allen J.D. et al. (1999) Cancer Res. 59: 4237-41. 4. Doyle L.A., et al. (1998) Proc Natl Acad Sci USA. 95: 15665-70. 5. Allikmets R., et al. (1998) Cancer Res. 58: 5337-9. 6. Miyake K., et al. (1999) Cancer Res. 59: 8-13. 7. Robey R.W., et al. (2001) Clin Cancer Res. 7: 145-52. Zhou S., et al. (2001) Nat. Medicine. 7: 1028.

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ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

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