StressMarq蚂蚁淘在线

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Clonality	Polyclonal
Isotype	IgG
Specificity	DetectsendogenouslevelsoftotalABCG2
CiteThisProduct	StressMarqBiosciencesCat#SPC-1317,RRID:AB_2713493
CertificateofAnalysis	A1:1000dilutionofSPC-1317wassufficientfordetectionofABCG2in10µgofmousekidneycelllyatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	ABCtransporterantibodyABC15antibodyABCG2antibodyABCG2antibodyABCG2_HUMANantibodyABCPantibodyATPbindingcassettesubfamilyG(WHITE)member2antibodyATPbindingcassettetransporterG2antibodyATP-bindingcassettesub-familyGmember2antibodyBCRPantibodyBCRP1antibodyBMDPantibodyBreastcancerresistanceproteinantibodyCD338antibodyCDw338antibodyCDw338antigenantibodyEST157481antibodyGOUT1antibodyMGC102821antibodyMitoxantroneresistanceassociatedproteinantibodyMitoxantroneresistance-associatedproteinantibodyMRXantibodyMultidrugresistanceeffluxtransportATPbindingcassettesubfamilyG(WHITE)member2antibodyMXRantibodyMXR1antibodyPlacentaspecificATPbindingcassettetransporterantibodyPlacentaspecificMDRproteinantibodyPlacenta-specificATP-bindingcassettetransporterantibodyUAQTL1antibody
CellularLocalization	Cellmembrane,Mitochondrionmembrane
AccessionNumber	NP_004818.2
GeneID	9429
SwissProt	Q9UNQ0
ScientificBackground	ABCG2(BCRP1/ABCP/MXR)isamemberoftheATP-bindingcassettetransporterfamilythatfunctionsasATP-dependenttransportersforawidevarietyofchemicalcompoundsandareassociatedwithdrug-resistanceincancercells.ABCG2isaheavilyglycosylatedtransmembraneproteinwithsixtransmembranespanningregionsconsistentwithitfunctioningasahalf-transporter.TheABCfamilycanexistaseitherfull-lengthtransportersorashalf-transportersthatformfunctionaltransportersthroughhomo-orheterodimerization.Originallythoughttoplayaroleindrugresistanceduetoitsresistancetoanumberofchemotherapeuticdrugs,ABCG2hasalsobeenfoundinthekidney,andstemcellpopulations.StudiesalsosuggestthatitcanbeusedasacellsurfaceMarkertoidentifyhematopoieticstemcellswithinthebonemarrowfractionoflineagenegativecells.TheexpressionofABCG2appearsgreatestonCD34-cellsandisdownregulatedwiththeacquisitionofCD34onthecellsurface.
References	1.ZhouS.,etal.(2001)Nat.Medicine.7:1028. 2.DoyleL.A.andRossD.D.(2003)Oncogene.22:7340-58. 3.AllenJ.D.etal.(1999)CancerRes.59:4237-41. 4.DoyleL.A.,etal.(1998)ProcNatlAcadSciUSA.95:15665-70. 5.AllikmetsR.,etal.(1998)CancerRes.58:5337-9. 6.MiyakeK.,etal.(1999)CancerRes.59:8-13. 7.RobeyR.W.,etal.(2001)ClinCancerRes.7:145-52.ZhouS.,etal.(2001)Nat.Medicine.7:1028.

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ATTO633

Overview:

Highfluorescenceyield
HighthermalandphotostABIlity
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

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