StressMarq蚂蚁淘在线

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects~40kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-900,RRID:AB_2706599
CertificateofAnalysis	A1:250dilutionofSPC-900wassufficientfordetectionofA6(pTyr309)in10µgofhumanglioblastomacelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	A6antibody,A6proteintyrosinekinaseantibody,ProteinA6antibody,Proteintyrosinekinase9antibody,PTK9antibody,PTK9proteintyrosinekinase9antibody,TWF1antibody,twf1antibody,TWF1_HUMANantibody,Twinfilin1antibody,Twinfilinactinbindingprotein,homolog1antibody,Twinfilinantibody,Twinfilin-1antibody
ResearchAreas	CellSignaling,Cytoskeleton,MICROFILaments
CellularLocalization	Cytoplasm,Cytoskeleton
AccessionNumber	NP_002737
GeneID	5756
SwissProt	Q12792
ScientificBackground	A6appearstobeanactin-bindingproteininvolvedinmotileandmorphologicalprocesses.Itinhibitsactinpolymerization,probablybysequesteringG-actin.Bycappingthebarbedendsoffilaments,italsoregulatesmotility.Itseemstoplayanimportantroleinclathrin-mediatedendocytosisanddistributionofendocyticorganelles.

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ATTO655

Overview:

Highfluorescenceyield
HighthermalandphotostABIlity
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

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