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CatalogNo.:56462(cloneS151-3)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

GABAisthemajorinhibitoryneuro-transmitterinthevertebratebrain.GABAreceptorsconsistoffivesubunitsthatformachlorideionchannel.Subunitfamiliesα,β,γ,andδhavebeenstudiedextensively;πandεhavebeenidentifiedrecently.

StudiesofrecombinantGABAreceptorshaveshownthatindividualsubunitsandtheirsubtypesconferdifferentsensitivitiestoGABAreceptormodulators.SubunitsubtypesaredifferentiallyexpressedthroughoutdevelopmentandindifferentCNSregions,reducingthetotalnumberofpossIBLeisoformsthatcanbeformedindifferentbrainregionsandinindividualcells.

ThisantibodyrecognizesmouseandratDeltaGABA-Areceptor.

Immunoblotting:useat1-10ug/ml.Abandof~55kDaisdetected.

RatcerebellarlysateImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/ml

Immunofluorescence:useat1-10ug/ml

Rathippocampalneuronsurface(S)andtotal(T)proteinimmunofluorescentstaining.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Ratbrain.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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