CatalogNo.:2239

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

AnovelproteinthatcauseschromatincondensationandDNAfragmentationhasbeendesignatedApoptosisInducingFactor(AIF).AIFlocalizesinmitochondriaandtranslocatestothenucleuswhenapoptosisisinduced.Thiseventisfollowedbythereleaseofchytochromecandcaspase-9frommitochondria.AIFishighlyconservedbetweenhumanandmouseandwidelyexpressedindifferentcelltypesandtissues.

Antigen:Peptidecorrespondingtoaa109-122attheN-terminusofhumanAIF.

HostSpecies:Rabbit

Preservatives:0.02%sodiumazide

ThisantibodyrecognizeshumanAIF(67kDa).

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Immunoblotting:useat1-10ug/ml.

WesternblotanalysisofAIFinK562celllysatewithAIF(NT)antibodyat1μg/ml.

Immunocytochemistry:useat2-5ug/ml.

ImmunocytochemicalstainingofAIFinJurkatcellswithAIF(NT)antibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:K562ofJurkatcellsorwholecelllysates.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.