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Format:Sterilefiltered,lyophilized.

Purification:Chromatographic

RecombinantHumanVEGFproducedin

E.coliisadouble,non-glycosylated,polypeptidechaincontaining165aminoacidswithamolecularmassof38,231daltons.ThisproducthasfullBIOLOGicalactivity.

Source:EscherichiacoliStABIlizers:NonePreservatives:None

Purity:Greaterthan98%byRP-HPLC,anion-exchangechromatography;reducingandnon-reducingSDS-PAGE,silverstain.

Dimers/Aggregates:<1%bySDS-PAGE

BiologicalActivity:Thebiologicalactivityisdeterminedbythedose-dependentstimulationofthe

proliferationofhumanumbilicalveinendothelialcellsusingaconcentrationof1.0to8.0ng/ml.

Endotoxin:<0.1ng/ug

ProteinContent:Determinedspectrophotometricallyat280nm(extinctioncoefficient0.2875)andbyRP-HPLCanalysisagainstacalibratedstandard.

Reconstitutethelyophilizedproductinsterilewaternotlessthan100ug/ml.

Lyophilizedproductisstableatroomtemperaturefor3weeks.Storelyophilizedproductdesiccatedat-20°C.Uponreconstitution,storeat4°Cfor2-7daysoraliquotandstoreat-20°C.Forlong-termstorageaddeitherHSAorBSAto0.1%.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

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SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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