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Formulation:Sterile-filteredsolutionin20mMTris,pH7.5,2mMEDTA,and20%glycerol.Purifiedbyproprietarychromatographictechniques.

PCNAislocatedinthenucleusandisacofactorofDNApolymerasedelta.

PCNAactsasahomotrimerandhelpsinprocessingofleADIngstrandsynthesisduringDNAreplication.InreactiontoDNAdamage,PCNAisubiquitinatedandparticipatesintheRAD6-dependentDNArepairpathway.TwotranscriptvariantsencodingthesameproteinhavebeenfoundforPCNAgene.PCNAisexpressedduringlateG1phase,Sphaseofmitosis,andpersistsuntiltheendoftheMphase.

PCNAcanbeinducedbyUVirradiation.

RecombinantHumanPCNAproducedin

E.coliisasinglenon-glycosylatedpolypeptidechaincontaining261aminoacidswithamolecularweightof28.8kDa.

Source:EscherichiacoliPurity:Greaterthan95%asdeterminedbySDS-PAGE.Accessionnumber:P12004Aminoacidsequence:

MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS.

Althoughstableat4oCfor4weeks,productisbeststoredatorbelow-20oC.Additionofacarrierprotein(suchas0.1%HSAorBSA)isrecommendedforlong-termstorage.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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