ORDERINGINFORMATION

CatalogNo.:43057

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Mutationsinp95/NBS1geneareassociatedwithNijmegenbreakagesyndrome,anautosomalrecessivechromosomalinstABIlitysyndromecharacterizedbymicrocephaly,growthretardation,immunodeficiency,andcancerpredisposition.TheencodedproteinisamemberoftheMRE11/RAD50double-strandbreakrepaircomplexwhichconsistsof5proteins.ThisgeneproductisthoughttobeinvolvedinDNAdouble-strandbreakrepairandDNAdamage-inducedcheckpointactivation.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine343(S-L-S(p)-Q-G)derivedfromhumanp95/NBS1.

HostSpecies:Rabbit

Accessionno.:O60934,NP_002476.2

Specificity:Thisantibodydetectsendogenoushumanp95/NBS1onlywhenphosphorylatedatserine343.

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~95kDaisdetected.

Detectionofp95/NBS1(phospho-Ser343)inextractsofHeLacellsuntreatedortreatedwithhydroxyurea.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.