ORDERINGINFORMATION

CatalogNo.:43022

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

c-Junincombinationwithc-Fos,formstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunction

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine93(T-T-T(p)-P-T)derivedfromhumanc-JunandconjugatedtoKLH.

Accessionno.:P05412,NP_002219.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatthreonine93.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Detectionofc-Jun(phospho-Thr93)inextractsofHeLacellsuntreatedortreatedwithPMA.

Detectionofc-Jun(phospho-Thr93)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.