ORDERINGINFORMATION

CatalogNo.:43065

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Bcl-2proteinsuppressesapoptosisinavarietyofcellularsystemsincludingfactor-dependentlymphohematopoieticandneuralcells.ItregulatescelldeathbycontrollingthemitochondrialmembranepermeABIlityandappearstofunctioninafeedbackloopsystemwithcaspase.Bcl-2inhibitscaspaseacativityeitherbypreventingreleaseofcytochromecfromthemitochondriaand/orbybindingtoapoptosis-activatingfactor(Apaf-1).ThepredictedmolecularweightofBcl-2is~26kDa.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine70(R-T-S(p)-P-L)derivedfromhumanBcl-2andconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanBcl-2onlywhenphosphorylatedatserine70.

Accessionno.:P10415,NP_000624.2

APPLICATIONS

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofBcl-2(phospho-Ser70)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.