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productdatasheet
CatalogNo.:12530(cloneS117-9)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
MethylationofDNAatcytosineresiduesisimportantinregulationofgeneexpression.HypermethylationofCpGislandsintumorsuppressorgenesorhypomethylationofbulkgenomicDNAmaybelinkedtodevelopmentofcancer.ThreefamiliesofmammalianDNAmethyltransferasegeneshavebeenidentified:DNMT1,DNMT2,andDNMT3.TheDNMT3familymembers,DNMT3aandDNMT3b,arestronglyexpressedinembryonicstem(ES)cells,buttheirexpressionisdownregulatedindifferentiatingEScellsandislowinadultsomatictissue.StudiesshowthatDNMT3LregulatestheactivityofDNMT3aandDNMT3bandstimulatestheircatalyticactivities.DNMT3Lhasbeenlinkedspecificallytotheprocessofcarcino-genesisthroughitsroleinnuclearprogramming.
BAA95556).
HostSpecies:MouseAntibodyClass:IgG1ThisantibodyrecognizeshumanDNMT3L.Itdoesnotcross-reactwithDNMT3aorDNMT3b.
Immunoblotting:useat1-10ug/ml.Abandof~45kDaisdetected.
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:LysateofCOScellstransientlytransfectedwithFlag-taggedDNMT3L.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
QEDBioscience,Inc.10919TechnologyPlace,SuiteC
SanDiego,CA92127
TollFree800.929.2114
Phone858.675.2405
Fax858.592.1509
info@qedbio.com
Visitourwebsiteforadditionalproductinformationandtoorderonline.
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QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司。能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。
