神经组学/平板涂层缓冲液-CB1/KF17336-1/1 L

价格
¥4380.00
货号:KF17336-1
浏览量:127
品牌:Neuromics
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商品描述

Type:CoatingBuffer

 

Applications:E

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

Format:liquid

 

Thisuniqueplatecoatingbufferisforusewithantibodiesandantigens.ThepHandionicstrengthofthisbufferhavebeenspecificallyformulatedtocreateanoptimalcoatingenvironmentwhichmaximizestheadsorptionefficiencyofmostantibodiesandproteinantigensontopolystyreneplatesurfaces. 
    
ThisbufferalsostABIlizescoatedproteinsbymaintainingtheirtertiarythree-dimensionalstructureduringtheadsorptionprocess.Bystabilizingtheadsorbedprotein,antigenicregionsarepreserved,allowingforgreaterbindingreactivitywiththedetectionmolecule,therebyenhancingthespecificsignal.
    

Bygeneratingahigherspecificsignal,alowerconcentrationofcoatproteinmaybeneededwhenthisbufferisused,therebysavingvaluablereagents.Thereisafinitequantityofproteinthatmaybecoatedeffectivelyontomicrotiterplates. Useofproteinconcentrationsinexcessoftheoptimalrangewillresultinsurfacesaturationandclumpingontheplatesurface. Thisproduceslowerspecificsignals,andhighernonspecificsignals.

Thecoatingbuffercontainsanantimicrobialagenttoretardbacterialgrowthduringtheplatecoatingprocess,allowingtheproceduretobeperformedatroomtemperature.Dependingontheactivityofthecoatedprotein,platesmaybestoredat2°-8°C(orevenatroomtemperatureforhardyproteins)forseveralmonths,orevenyears.

 

 

 

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