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Type:CoatingBuffer
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid
Bygeneratingahigherspecificsignal,alowerconcentrationofcoatproteinmaybeneededwhenthisbufferisused,therebysavingvaluablereagents.Thereisafinitequantityofproteinthatmaybecoatedeffectivelyontomicrotiterplates. Useofproteinconcentrationsinexcessoftheoptimalrangewillresultinsurfacesaturationandclumpingontheplatesurface. Thisproduceslowerspecificsignals,andhighernonspecificsignals.
Thecoatingbuffercontainsanantimicrobialagenttoretardbacterialgrowthduringtheplatecoatingprocess,allowingtheproceduretobeperformedatroomtemperature.Dependingontheactivityofthecoatedprotein,platesmaybestoredat2°-8°C(orevenatroomtemperatureforhardyproteins)forseveralmonths,orevenyears.