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ImmunoglobulinD(IgD)isanantibodyisotypethatisfoundprimarilyonmatureB-cellsaspartoftheB-cellreceptor(BCR)complex.ClusteringoftheBCRduetoantigenbindingleadstoactivationofB-cellsthatcanresultinanumberofoutcomesincludingproliferation,differentiation,andtolerance.
TheABIlitytoactivateB-lymphocytesusingpolyclonalantiserarecognizinganti-IgDisusefulforthestudyofB-cellfunction(Finkelman,etal.,1985;Nguyen,etal.,2014).Anti-IgDisparticularlysuitedforthisapplicationbecausesolubleIgDispresentinextremelylowlevelsinserum(<0.25%oftotalimmunoglobulin)andwillnotinterferewithB-cellactivationinawholeblood-basedorinvivosetting.(Incontrast,highlevelsofcirculatingIgMwilltypicallyblockB-cellactivationbyanti-IgMinwholeblood.)SuchamethodologycanbeusedtorapidlytesttheefficacyofB-cellinhibitoryagentsinacellular,exvivo,orinvivocontext(Coffey,etal.,2012).
Format: 1mlpervial
Host: Goat
Reactivity: MouseimmunoglobulinCδchain
Buffer: None
Preservative: Preservative-freeforinvivoapplication,0.2μmsterile-filtered
Storage: Shorttermat2-8°C.Forlongtermstorage,-80°Cisrecommended.Avoidmultiplefreeze-thawcycles.Shouldbekeptsterile.Canbere-filteredifnecessary.
Expiration: Seeviallabel
How_To_Use:
IgDcross-linkingonBcellsisanidealmethodfortheassessmentofacutepolyclonalBcellactivationacrossmultiplestrainsofmice.Followinganti-IgDinjection(100μl/mouse,i.v.),peripheralBcellsarerapidlyactivatedasevidencedbyup-regulationofCD86andCD69.ActivatedBcellswillsecreteeffectorcytokines(IL-4)andacquireeffectorfunction(secretionofIgEantibodies)within5-7days.Anti-mouseIgDantibodycanalsobeusedtoinducefunctionalBcellsinvitro.ThismodelsystemisidealforspecificexaminationofkeypathwaysandplayersinthefunctionalactivationofperipheralBcellsinvivo.
