Specifications

“The VU-1 antibody from LifeSensors is the best anti-ubiquitin antibody that we have found after trying more than ten different anti-ubiquitin antibodies.”– Aldrin Gomes, Ph.D.Associate ProfessorUniversity of California, Davis

Ubiquitin (Ub), a highly conserved, 8 kDa polypeptide present in all eukaryotic cells, is conjugated to the ε-amino group of lysine residues in the target protein through the sequential action of three enzymes, an E1 Ub activating enzyme, an E2 conjugating enzyme, and an E3 ligase. In addition, the seven lysines within Ub itself can serve as Ub acceptors leading to the formation of polyubiquitin chains. Among these K48 and K63 linkages are well characterized. K48-linked polyubiquitylation targets proteins for proteasomal degradation whereas K63 linkages regulate signaling events, receptor endocytosis and immune responses. Polyubiquitin linkages at other lysines are less prominent and their physiological role remains largely unknown.VU-1 is a monoclonal antibody that has been shown to recognize free ubiquitin, K48-, K63-, K11- and linear chains on Western blot. Other linkages have not been specifically tested. VU-1 is an excellent antibody for immunostaining applications generating robust ubiquitin detection and low background with an ability to detect subcellular ubiquitin localization.

Info

Species	Murine
Purity	>90%
Isotype	IgG1
Production	Cell Culture
Concentration	0.4-0.5 mg/mL
Stability	Stable for weeks at +4°C. Sodium azide can be added to an antibody preparation to afinal concentration 0.02% (w/v).
Storage	For long term storage (6 to 12 months) store at -20°C. Avoid multiple freeze/thaw cycles.

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用