Specifications

The SUMO3-CHOP2-Reporter DeSUMOylation Assay consists of human SUMO3 fused to a reporter enzyme, as well as a separate substrate for the reporter enzyme. When fused to SUMO3, the reporter is rendered catalytically inactive. Following cleavage of the SUMO3-reporter system by the isopeptidase, free (and now active) reporter subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.

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Contents:

• SUMO3-CHOP2-Reporter System: 1 x 375 μL (4 μM)
• SENP2 core (Control Isopeptidase): 2 x 10 μL (2 μM)
• Reporter Substrate 2: 1 x 30 μL (25 μM)
• Control Fluorophore 2 :1 x 20 μL (1 mM)

Info

Storage

-80° C, avoid freeze/thaw cycles

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用