PROteolysis TArgeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase, thereby leading to ubiquitination and subsequent degradation of the target protein. LifeSensors has developed a high-throughput plate-based method to directly monitor PROTAC-mediated ubiquitination and degradation of target proteins. This method is rapid, efficient, and quantitative and works with endogenous proteins and cell lines. There is no need for stable cell line production or tagging your target protein and creating artifacts.
Components:
| Coated plates: | One pre-coated and blocked 96-well strip plate (Store at -80°C) |
| Blocking concentrate (BC): | 12 mL of 5X blocking agent for antibody dilution (Store at 4°C) |
| Plastic plate seals: | Two plate seals |
| Detection reagent 1 (DR1): | 1.0 mL vial of DR1 reagent (Store at 4°C) |
| Detection reagent 2 (DR2): | 1.0 mL vial of DR2 reagent (Store at 4°C) |
| MG132: | 25 µL vial (10 mM in DMSO; Store at -20°C) |
| PR-619: | 25 µL vial (22 mM in DMSO; Store at -20°C) |
| 1,10-phenanthroline: | 100 µL vial (500 mM in DMSO; Store at -20°C) |
