Specifications

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3].  Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods.  Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro.  Unlike others, LifeSensors’ TAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Info

Purity	≥ 95% by RP-HPLC
Molecular weight	9133.5 Da
Physical State	Liquid, PBS
Quantity	100ug
Solubility	>8mg/mL
Wavelength Maxima	Ex: 540 nm, Em: 578 nm
Storage	-80°C. Avoid repeated freeze/thaw cycles

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用