Specifications

This kit allows you to detect whether your target protein is ubiquitylated and determine the linkage type of the ubiquitin chain.

Determining the linkage of polyubiquitin on target proteins is challenging. The traditional methods are either through Mass Spectrometry which is cumbersome or immunoblot with linkage specific antibodies, which are unreliable. LifeSensors has developed a more definitive method for demonstrating the ubiquitylation linkage of a protein, which is to couple immunoprecipitation of polyubiquitylated protein with digestion by a linkage specific deubiquitylase prior to immunoblot analysis. An increased signal for the unmodified substrate or a decreased signal of polyubiquitylated substrate at high molecular weight after K48/K63 specific DUB treatment is a clear indication that the protein was K48/K63 ubiquitylated. We have built this kit around the use of Magnetic Tandem Ubiquitin Binding Entities (TUBEs) which bind to all ubiquitin chain linkages. Magnetic-TUBEs are TUBE moieties directly coupled to magnetic beads, for the identification and characterization of polyubiquitinated proteins by western blotting and/or downstream proteomic studies.  Magnetic-TUBEs facilitate convenient “one-step” pull-down of polyubiquitinated proteins.

This kit includes the following DUBs:

• A Lys48 linkage specific DUB – 25 µg, 10 µM

• A Lys63 linkage specific DUB – 35 µg, 10 µM

• A broad spectrum DUB – 25 µg, 10 µM

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用