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Specifications
Todate,detectingchangesintheubiquitylationofspecificsubstrateproteinsinresponsetoexternalstimuli,e.g.stress,cytokineexposure,drugcandidatetreatment,etc.,hasbeenalong,labor-intensiveprocessinvolvingimmunoprecipitationfollowedbygelelectrophoresisandWesternblotanalysis. Thismethodislowthrough-put,resourceintensive,andonlysemi-quantitativeatbest. WehavedevelopedtheUbiQuant™Skitasafacile,robust,andquantitativealternativetoIP/WBanalysis.BuiltonLifeSensorsUbiQuantplatform,thesystemcapturesubiquitylatedproteinsinthewellsofamicrotiterplateandthenusesanantibodytotheproteinofinterest(eithernativeortagged)toquantitatetheamountoftheproteinbound.
Note: Thisproductonlyincludesthecoatedplate.See UbiQuantSKit foracompleteexperiment(plate,anibodies,detectionreagent,blockingagent).
Selection
- 96or384wellplates
- ModularorStripPlates
Storage
- Longterm:-80Cforupto1year
- Shortterm:4Cforupto4weeks


