Specifications

K27-linked di-ubiquitin (Ub2) is a diubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 27 on the proximal ubiquitin. The product is generated using chemical ligation. It is a useful substrate for identifying and characterizing deubiquitinating enzymes that cleave the K27-linkage and for structural and binding studies of ubiquitin chain recognition by ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs).

Info

Purity	> 95% by HPLC
Molecular Weight	17,112 Da
Physical State	Lyophilized powder
Recommended Buffer	DMSO or 0.05% HOAc
Buffer	20 mM Tris, pH 7.5, 0.15 M NaCl, 1 mM EDTA
Storage	-80°C, avoid cycles of freeze/thaw
Stability	1 year under recommended storage conditions

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