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蚂蚁淘在线 / 品牌中心 / Life Sensors / LifeSensors/UC101 E3LITE Customizable Ubiquitin Ligase Kit-384 well plate/UC101 E3LITE Customizable Ubiquitin Ligase Kit-384

LifeSensors/UC101 E3LITE Customizable Ubiquitin Ligase Kit-384 well plate/UC101 E3LITE Customizable Ubiquitin Ligase Kit-384

价格

￥13100.00

货号：UC101E3LITECustomizableUbiquitinLigaseKit-384wellplat

浏览量：127

品牌：Life Sensors

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ThissimpleandrobustassayallowsforquantificationofE3ubiquitinligaseactivity. ThekitemploysaproprietaryreagenttocapturepolyubiquitinchainsformedinanE3ligasedependentmanner.DeterminationoftheamountofproductformedisaquantitativemeasureoftheE3ligaseactivity,andthedetectionstrategydoesnotrequireadditionaltaggingorlabelingofeitherubiquitinortheligationmachinery.AnalyzingE3ligaseactivityenablesenzymecharacterizationaswellascompoundscreeningformodulatorsofubiquitylation,anemergingfocusinthedrugdiscoveryfield.Thisassayhasbeenvalidatedforhighthroughputscreening(HTS)andhasbeenemployedinthediscoveryofnovelcompoundsthatselectivelymodulateE3ligasefunction.*

TheE3LITECustomizableUbiquitinLigaseKitisflexIBLebydesign,essentiallyprovidingasingularplatformforthefocusedinvestigationofanyE2/E3enzymepair,aswellasanyparticularpolyubiquitinlinkagetype.Insupportofthisflexibleplatform,LifeSensorsoffersthewidestarrayofreagentsonthemarkettomeetyourresearchneeds.Thekititselfcanbeassembledwithanyoneofmorethantwenty-fourE2conjugationenzymes,aselectionrepresentingmembersfromeachenzymeclass.FormoreE2enzymes,assembleapanelof(4)fromE2Selectionpanel.Therecombinantubiquitincomponentofthiskitcanbewild-type,variantsthatspecificallyformK48linkagetypes,orvariantsthatformlinkagesonlythroughoneofthesevenpossiblesurfaceexposedlysines.

*Platesmustbeusedwithin30daysofreceipt.

ThisproductisprotectedbyoneormoreUSorForeignpatents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplytotheseterms.

PriceshownisfortheoneE2enzymeincludedinthekit.Additional(4)E2panelcanbepurchasedfor$195.ToordermoreE2s,pleaseinquireabouttheprice. Email info@lifesensors.com formoreinformation,includingcommercialavailABIlity.

Info

KitContents:

UbiquitinE1ActivatingEnzyme
ChoiceofUbiquitinE2ConjugatingEnzyme(s)
E1-E2-E3Control
ChoiceofRecombinantUbiquitin
DetectionReagent1
LinearPolyubiquitinControl
(2)modular96-wellplatescoatedwithLifeSensorsproprietaryreagent

Storage	Allcomponentsshouldbestoredat-80°C.
Stability	Allcomponents,exceptformicrotiterplates,arestableforatleast2monthswhenstoredat-80°C. Platesmustbeusedwithin30days.

Theselectionofappropriatestreptavidinreagentiscriticaltogeneratingarobustsignal.Wedonotrecommendusingavidin-horserADIshperoxidase(HRP)conjugates.

Applications

QuantitativeAnalysis of UbiquitinE2ConjugationorE3LigaseActivity
DemonstrationofnovelE2orE3Activity
HighThroughputScreening(HTS)ofantagoNISToragonistsofligaseactivity
Monitorenzymeactivityduringpurification

Benefits

MonitorE3activityinsolutionphase,withtheE2ofyourchoice
Plate-basedformatisamenabletohigh-throughputscreening(HTS)
DetectionsystemprovidesrobustreadoutforE3ligaseactivity
E₃LITEassayutilizesnon-radioactivereportersubstrates
E₃LITEassaydoesnotrequireexcitationintheUVrange(reducingfalsepositiverate)

Documents

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用

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