Specifications

“TheVU-1antibodyfromLifeSensorsisthebestanti-ubiquitinantibodythatwehavefoundaftertryingmorethantendifferentanti-ubiquitinantibodies.”–AldrinGomes,Ph.D.AssociateProfessorUniversityofCalifornia,Davis

Ubiquitin(Ub),ahighlyconserved,8kDapolypeptidepresentinalleukaryoticcells,isconjugatedtotheε-aminogroupoflysineresiduesinthetargetproteinthroughthesequentialactionofthreeenzymes,anE1Ubactivatingenzyme,anE2conjugatingenzyme,andanE3ligase.Inaddition,thesevenlysineswithinUbitselfcanserveasUbacceptorsleADIngtotheformationofpolyubiquitinchains.AmongtheseK48andK63linkagesarewellcharacterized.K48-linkedpolyubiquitylationtargetsproteinsforproteasomaldegradationwhereasK63linkagesregulatesignalingevents,receptorendocytosisandimmuneresponses.Polyubiquitinlinkagesatotherlysinesarelessprominentandtheirphysiologicalroleremainslargelyunknown.VU-1isamonoclonalantibodythathasbeenshowntorecognizefreeubiquitin,K48-,K63-,K11-andlinearchainsonWesternblot.Otherlinkageshavenotbeenspecificallytested.VU-1isanexcellentantibodyforimmunostainingapplicationsgeneratingrobustubiquitindetectionandlowbackgroundwithanABIlitytodetectsubcellularubiquitinlocalization.

Info

Species	Murine
Purity	>90%
Isotype	IgG1
Production	CellCulture
Concentration	0.4-0.5mg/mL
Stability	Stableforweeksat+4°C.Sodiumazidecanbeaddedtoanantibodypreparationtoafinalconcentration0.02%(w/v).
Storage	Forlongtermstorage(6to12months)storeat-20°C. Avoidmultiplefreeze/thawcycles.

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用