Specifications

SUMOstar Fusion Protein TechnologyThe pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convenient endonuclease restriction sites, while mult-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin.This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

• pY-secSUMOstar vector (Cat.# 2161) — 20 ug

• SUMOstar Protease (Cat.# 4110) — 500 Units

• SUMOstar Protease control protein (Cat.# 5010) — 100 µg

• Anti-SUMOstar antibody (Cat.# AB7002) — 50 µg

Info

Fusion Tag                         His6-hSUMOstarPromoter                            AOX1Host                                       P. pastorisDNA                                       CircularStorage                                 Store vector components at -20°C, store protease and antibody components at -80°C, avoid repeated freeze-thaw cycles

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用