Specifications

TheE2SELECT™Profiling&SelectionKitallowsexplorationoffunctionalcompetencybetweenanE3ligaseofinterestacrossapaneloftwenty-fourE2conjugatingenzymes. Atthecoreoftheassayisamicrotiterplatepre-coatedwithaproprietaryreagentthatcapturespolyubiquitinchainsformedinanE3ligase-dependentreaction.CocktailsofE1,E2,andubiquitinareprovidedintheplatewithtwenty-fourE2spresentintriplicateindifferentwellsoftheplate.AdditionofanE3ligase,togetherwithATP,initiatesthegenerationofpolyubiquitin,whichisrecognizedandcapturedinthewells.Thecapturedpolyubiquitylatedproductisdetectedinachemiluminescent“sandwich”ELISA-likeassayandisaquantitativemeasureofE2-E3functionality.ThisproductisprotectedbyoneormoreUSorForeignpatents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplytotheseterms.

Info

Kitcontains:

• 1x96-wellplatepre-platedwithcocktailsofE1,E2,andUbiquitin. 24E2sareprovidedintriplicate.

• E1-E2-E3Control

• LinearPolyubiquitinDetectionControl

• DetectionReagent1

Storage	Storeat-80°C
StABIlity	Eachplateisindividuallyprepared.Usethekitwithinonemonth.

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