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Specifications
Linearpolyubiquitin,ormet1-linkedpolyubiquitin,ispolyubiquitinchainslinkedthroughtheC-terminusofoneubiquitinandtheN-terminusofanother.Linearpolyubiquitinplaysimportantrolesininflammatoryandimmuneresponses,particularlywithintheNFkappaBsignalingpathway.LifeSensors’linearpolyubiquitinantibodydisplayshighspecificityforlinearpolyubiquitinchainsandissuitableforimmunoblotdetectionoflinearubiquitinchainsfromtissueorcelllysates.Theantibodyisnotsuitableforimmunoprecipitationandhasnotyetbeentestedforimmunostainingapplications.LUB9specificallydetectslinearoverK11-,K48-andK63-linkedpolyubiquitin.Info
| Immunogen | Linearpolyubiquitin |
| Isotype | IgG2b(murine) |
| Purification | PurifiedfromcellculturesupernatentbyproteinAaffinitychromatography |
| Specificity | AntibodydetectpolyubiquitinlinkedthroughtheC-terminusofoneubiquitinandtheN-terminusofanother.Reactivityisindependentofspeciesduetothehighconservationofubiquitinacrosseukaryotes. |
| Suppliedas | 1.0-2.5 mg/mlin10mMphosphatebuffer,0.15MNaCl,pH7.4 |
| Quantity | 100µg;Sufficientfor>10Westernblotapplications |
| Storage | Storeat-20ºC.Avoidmultiplefreeze/thawcycles.Dilutedantibodycanbestoredat4ºCforuptothreeweeks. |


