Specifications

Fluorescence polarization substrate for deubiquitinating enzymes. The substrate consists of a 5-carboxytetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide where the Ɛ-amino group of Lys is linked to the C-terminus of ubiquitin.  The substrate allows continuous fluorescence polarization measurement of cleavage of the isopeptide bond between ubiquitin and the Ɛ-amino group of Lys. The rhodamine fluorophore minimizes interference from compounds and assay components that fluoresce at lower wavelengths.

Info

Solubility	10-100 nM
State	Dry powder
Purity	> 95% by RP-HPLC
Molecular Weight	9162 Da
Storage	-80°C . Avoid freeze/thaw cycles

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