Specifications

“Mylabhassuccessfullyusedtheagarose-TUBEs.LifeSensors’TUBEsareeasytouseandhighlyspecific.MylabislookingforwardtousingthelinkagespecificTUBEs.”

Prof.DavidWallach,TheWeizmannInstituteofScienceBasedonproteindomainsknowntopossessanaffinityforubiquitin,TandemUbiquitinBindingEntities(TUBEs)havebeendevelopedfortheisolationandidentificationofubiquitylatedproteins.TUBEsdisplayuptoa1000-foldincreaseinaffinityforpolyubiquitinmoietiesoverthesingleubiquitinbindingassociateddomain(UBA).Inaddition,TUBEsdisplayaprotectiveeffectonpolyubiquitinatedproteins,allowingfordetectionatrelativelylowabundance.Thesepropertieseffectively“capture”proteininitspolyubiquitinstate.

PolyubiquitinchainsarelABIle,particularlyincelllysates.SuccesswithAgaroseTUBEsisoftendependentontheinclusionofdeubiquitylase(DUB)inhibitorsinyourlysisbufferstopreventtheremovalofpolyubiquitinchainsandsubsequentlossofpolyubiquitylatedproteinsduringyourpurification.LifeSensorsoffersPR619(Cat.#SI9619),anon-selectiveinhibitorofcysteine-typeDUBs,and1,10-phenanthroline(Cat.#SI9649),aZn2+chelatorandpotentinhibitorofJAMM-typeDUBs,forinclusioninyourlysisbufferandprotectionofpolyubiquitinatedproteinsduringpurification.Pleaseseetheagarose-TUBEmanualforfurtherdetails.

TUBE2isbasedonUBA1fromhumanRAD23A.AgarosecoupledTUBEsallowsforefficientrecoveryofpolyubiquitinatedproteinsinasinglestep.PolyubiquitinatedproteincaneasilybeseparatedfromTUBEsforproteomicstudiesorforidentificationbywesternblotting.ThisproductisprotectedbyoneormoreUSorForeign patents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplywith theseterms.

Info

Size	1mLofa50%slurry
Buffer	PBS,pH7.2,20%ethanol
RecommendedConcentration	10-20µLofresinin500µLlysate(seemanual)
PhysicalState	Agarose-TUBE2isprovidedasa50%slurry
Storage	-20°C.Storagebelowthistemperaturemayresultinfreezingand/orlossoffunction.

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用