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Specifications
13Cand15N,heavy-labeledubiquitinprovidesastablestandardforubiquitinstructuralstudybyNMR.TherecombinanthumanubiquitinispurifiedfromE.coliexpressingthehumanubiquitingene.Thecellsaregrowninmediawherethesolenitrogensourceis15N-labeledammoniumsulfateandthesolecarbonsourceis13C-labeledglucose.Therecombinanthumanubiquitinispurifiedwithoutheatingbyaproprietaryprocedurethatpreservesthenativestructureoftheprotein.ThepurifiedproteinishomogenouswhenanalyzedbyoverloadedSDSgels.Thepoolsofpurifiedproteinsolutionareextensivelydialyzedagainstwaterandlyophilized.
Info
| Purity | 98%byRP-HPLC |
| Protein | Ubiquitin |
| Atom%Enrichment | 13C(99%),15N(98%) |
| Physicalstate | Lyophilizedpowder |
| Quantity | 5mg,10mg |
| Solubility | >30mg/mLinaqueoussolution |
| Storage | -20°C |


