Description
TheantibodyhasaprovenstrongBiotin-Streptavidin/HRPstainingata1/4000-1/6000dilutioninratamygdala,cortex,andsuprachiasmaticnucleus.
Thespecificityoftheantiserumwasexaminedbysolublepre-adsorptionwiththepeptidesinquestionatafinalconcentrationof10-5M.VIPimmunolabelingwascompletelyabolishedbypre-adsorptionwithVIP.Pre-adsorptionwiththefollowingpeptidesresultedinnoreductionofimmunostaining:Secretin,gastricinhibitorypolypeptide,somatostatin,glucagon,insulin,ACTH,gastrin34,FMRF-amide,ratGHRF,humanGHRF,peptidehistidineisoleucine27,ratpancreaticpolypeptide,motilin,peptideYY,substanceP,neuropeptideY,andCGRP.
ThehistochemicalantibodyforVIPisgeneratedinarabbitagainstporcineVIPconjugatedtobovinethyroglobulinwithcarbodiimide.Theantibodyisprovidedas100µLoflyophilizedwholeserum,and0.09%sodiumazide.
PhotoDescription:IHCimageofratcortex(above)andlowmagnificationimageofratstriatumandamygdalastainingforVIP(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith:Avian(Bird),Bat,BubalusBubalis(Buffalo),Bullfrog,Canine,Cat,Chick,Chicken,Dog,Dove,Fish,Fowl,Fox,Frog,Gilt(Pig),Goldfish,GuineaPig,Hamster,Hedgehog,Hen,Human,Lizard,Mink,Mole,Mollusk,Monkey,Mouse,Mudpuppy(NecturusMaculosus),Ovine,Pig,Pigeon,Platypus,Possum,Rabbit,Raccoon,Ram,Rat,ScorpaenaPorcus(Fish),SeaLamprey,Sheep,Shrew,Snake,Sparrow,SparusAurata(Fish),Squirrel,Starfish,StingRay,SuncusMurinus,Tadpole,Turkey,Turtle,Worm(Spionidae),ZebraFinch,Zebrafish
AvailABIlity:InStock
PreabsorptionControl:AvailableatSigmaAldrich,Catalog#V6130
AlternateNames: Vasoactiveintestinalpolypeptide;VIPpeptides,anti-VIP
GeneSymbol/ID,Accession#: VIP,7432
RRID: AB_572270
