Theantibodyproducesastrongpostive labelingofLHRH/GnRHatdilutionof1/2,000-1,4,000usingbiotin-streptavidin/HRPinrathypothalamus(medianeminence).Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.

TheaboveimageisofLHRH(GnRH)terminalsinratmedianeminence,ryostatsections,ABC/DABdetection.

Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith5µgofLHRHpermLofdilutedantiserum.

PhotoDescription:IHCimageofstainingforLNRH/GnRHintheratmedianeminence.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:4000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

ReactsWith:Bat,Carp,Catfish,Chick,Chicken,ClariasBatrachus(Catfish),Fish,Frog,Frog(GreenTreefrog),Gerbil,GuineaPig,GuineaPig,Hagfish,Hamster,Human,Lizard,MoleRat,Monkey,Mouse,Mudpuppy(NecturusMaculosus),Octopus,Pig,Rat,Salamander,Snail,Snail(BullaGouldiana),Squid,Trout

AvailABIlity:InStock

AlternateNames: Gonadotropin-ReleasingHormone;Gonadorelin;LuliberinI;GonadoliberinI;progonadoliberin-1;GRH,anti-LHRH

GeneSymbol/ID,Accession#: GNRH1,2796

 RRID: AB_572248