Theantibodyhasaprovenstrongbiotin-streptavidin/HRPstainingata1/1000-1/2000dilutioninratglobuspallidusandspinalcord.

Stainingiscompletelyeliminatedbypretreatmentwith50µgofLeucineEnkephalinpermLofdilutedantiserum.Pretreatmentwith50µgofMethionineEnkephalinpermLofdilutedantiserumalsosignificantlyblocksstaining.

PhotoDescription:LowmagnificationIHCimageofleucineenkephalinstainingintheratglobuspallidus.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:2000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

Reacts With:Bird,Bovine,Bullfrog,Cat,Chick,Dog,Fish,Frog,GuineaPig,Hamster,Human,Lungfish,Monkey,Mouse,Octopus,Pig,Pigeon,Rat,Ray,Shark,Sheep,Starling,Three-SpinedStickleback,Turtle,ZebraFinch

AvailABIlity:InStock

AlternateNames: Enkephalin(Leu+Met);Proenkephalin-A;enkephalinA;preproenkephalin,anti-LeucineEnkephaline,LeuEnk 

GeneSymbol/ID,Accession#: PENK,5179

RRID: AB_572247