TheImmunoStarSEROtoninantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamusandspinalcordusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/5000-1/10,000inPBS/0.3%TritonX-100-Bn/Av-HRPTechnique.
Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith100µgofserotonin/BSAconjugate.
PhotoDescription:LowmagnificationofIHCimageofneuronsstainingforthe5-HTgoatantibodyinthepontineregionoftheratbrain(top),andhighmagnificationoftheraphenucleus(bottom).Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%rabbitseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:7500for48hours,rabbitanti-goatsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Goat
Quantity/Volume:100µL
State:LyophilizedWholeSerum
AvailABIlity:InStock
ReactsWith:Cat,Cod,Cow,Crayfish,Dolphin,Fish,Frog(XenopusLaevis),GuineaPig,Hamster,Human,Mexicansalamander(Ambystoma Mexicanum),Monkey,Moth,Mouse,Pig,Rabbit,Ram(Sheep),Rat,Salamander,Snail,Tapeworm,Worm,Zebrafish
AlternateNames: anti-5-HTGoat
RRID: AB_572262
Immunogen:Serotonin
GeneSymbol:Spl
GeneID:104153
ImmunoStar肽对照目录向下滚动以查看我们肽控制的完整列表。对于快速订购，请在上面的搜索框中输入产品名称或产品ID号。您也可以访问我们的抗体目录。按产品名称排序按产品编号排序5-HT（5-羟色胺）/ BSA结合控制产品编号：200815-羟色胺的结合物对照旨在用于5-羟色胺抗血清的免疫吸附，目录号为20079和20080。$ 125.00 5-HT转运蛋白肽控制产品编号：243325-HT转运蛋白的肽对照旨在用于5-HT转运蛋白抗血清的免疫吸附，货号24330。$ 75.00 5-HT2A受体肽控制产品编号：243335-HT2A受体的ImmunoStar肽对照用于5-HT2A受体抗血清的免疫吸附，货号24288。$ 75.00 C-FOS肽控制产品编号：24338C-FOS的肽对照旨在用于C-FOS抗血清的免疫吸附，货号26209。$ 75.00 DBH（多巴胺-β-羟化酶）蛋白控制-不再可用产品编号：24339根据抗体规格书稀释的DBH（多巴胺-β-羟化酶）抗血清的预吸附，用10 µg / ml纯化的DBH肽免疫原进行，可以完全阻断DBH免疫标记。$ 0.00 GluR1（离子型谷氨酸受体）肽对照产品编号：24440GluR1的肽对照旨在用于GluR1抗血清的免疫吸附，目录号24439。$ 75.00 神经激肽1受体（NK1R）肽对照产品编号：24340Neurokinin 1 Receptor的肽对照旨在用于Neurokinin 1 Receptor抗血清的免疫吸附，目录号20060。$ 75.00 nNOS：C端肽控制产品编号：24337nNOS（C末端）的肽对照用于nNOS（C末端）抗血清的免疫吸附，目录号24287。$ 75.00 nNOS：N末端肽控制产品编号：24447nNOS（N末端）的肽对照用于nNOS（N末端）抗血清的免疫吸附，目录号24431。$ 75.00 阿片受体-Mu肽控制产品编号：24335Mu阿片样物质受体的肽对照旨在用于Mu-阿片样物质受体抗血清（目录号24216）的免疫吸附。$ 75.00
