- 外显子-DNA-PS：从血浆和血清中分离循环和EV相关DNA
- 生物化学
- Exo准备
- 细胞增殖和分离分析
- 外显子DNAc PS：从血浆和血清中分离循环和EV相关DNA
- 尿液中冻干纯化外体
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 单克隆抗体
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- Exo试验：即用ELISA定量试剂盒
- RNA碱性试剂盒
- 冻干纯化血浆外体
- 氧化应激
- 冻干纯化血清外体
- 唾液外体的冻干纯化
- ELISA免疫平板
- 不同细胞系外体的冻干纯化
- 纯EVs：大小排阻色谱柱
- 肿瘤总RNA：肿瘤外体免疫捕获和RNA提取试剂盒
ExoTESTisasandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationinhumanserum,plasmaandurine.
Application:
ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.
Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.
SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.
Relatedproducts :
Exosomestandards
RNAextractionkit
Immunocaptureandisolationtools
HansaBioMed LLC是一家完全致力于外泌体（exosomes）研究工具开发的欧洲公司。公司以“为生命科学领域外泌体研究开发和商业化相关专利技术、试剂盒以及相关设备”为使命。HansaBioMed的研发生产基地位于爱沙尼亚首都塔林（Tallinn）科技园和意大利锡纳 (Siena)。 HansaBioMed公司可为全球生命科学市场提供最广泛的外泌体研究工具，是历史最悠久也是唯一一家只专注于提供高品质外泌体分离与分析相关产品的公司。目前公司主要提供的产品包括外泌体标准品（Exosome Standards）、外泌体结合抗体（Exosome binding Antibodies）以及特异性外泌体亚群富集试剂盒等。同时，HansaBiomed公司与来自学术界和产业界的国际知名研究团体合作，进一步发展其技术平台以及独有的生物标志物产品线。学术合作单位包括罗马的Istituto Superiore di Sanità研究所、美国哈佛大学和耶鲁大学以及里加的拉脱维亚大学等。-
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Sampletype | Plasma,serum,urine. |
Startingmaterial | Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Unfractionatedplasmasamplecanbedirectlyusedforcapture. Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Assaytype | Sandwichenzyme-linkedimmunoassay. |
Sensitivity | Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein. |
Productoverview | ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasma,serum,urineandcellculturesupernatant. ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies. |
Primaryantibody | α-CD9mouseantibody. |
Exosomestandard | LyophilizedexosomesfromCOLO-1Cellculturesupernatant(seedetails). |
Assayprotocol | Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:kitcontainsexosomestandardsforassaycalibration.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples. |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate | 12months. |