Cell Line Information:

Designations: 4T1Depositors: BA PulaskiGrowth Properties: adherentOrganism: Mus musculus deposited as mouseMorphology: epithelialOrgan: mammary glandStrain: BALB/cfC3HDisease: tumorTumorigenic: Yes4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can also be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy than most tumor models.

The Transfection Reagent:

4T1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 4T1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 4T1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, et al. Suppression of tumor-derived Semaphorin7A and genetic ablation of host-derived Semaphorin7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol. 2017 Nov;51(5):1395-1404. PubMed PMID: 29048670; PubMed Central PMCID: PMC5642386.

Features:

• Specifically optimized to deliver nucleic acids into 4T1 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

EZ Biosystems™是转染和基因表达产品及服务的全球供应商。我们的工厂位于马里兰大学帕克分校校园附近。公司致力于开发细胞类型特异性转染试剂，创造了世界上最大的细胞类型特异性转染试剂，可以在最常用的细胞系和原代细胞上进行最有效的转染。   产品 货号 规格 价格 Human Astrocyte (HA, NHA) Avalanche™  Transfection Reagent  EZT-HA00-1 0.5 ml $455.00 Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent  EZT-HA00-1 1.5 ml $795.00 Beta-TC-6 Cell Avalanche™ Transfection  Reagent (mouse pancreatic cancer cell) EZT-BETA-1 0.5 ml $455.00 Beta-TC-6 Cell Avalanche™ Transfection  Reagent (mouse pancreatic cancer cell) EZT-BETA-1 1.5 ml $795.00 H9c2(2-1) Cell Avalanche™ Transfection Reagent EZT-H9C2-1 0.5 ml $455.00 H9c2(2-1) Cell Avalanche™ Transfection Reagent EZT-H9C2-1 1.5 ml $795.00 Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent EZT-OCCM-1 0.5 ml $455.00 Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent EZT-OCCM-1 1.5 ml $795.00 Hep 3B Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-HEP3-1 0.5 ml $455.00 Hep 3B Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-HEP3-1 1.5 ml $795.00 Hep G2 Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)  EZT-HEPG-1 0.5 ml $455.00 Hep G2 Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)  EZT-HEPG-1 1.5 ml $795.00 Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)  EZT-HEPA-1 0.5 ml $455.00 Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)  EZT-HEPA-1 1.5 ml $795.00 HepG2/C3A Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-C3A0-1 0.5 ml $455.00 苏州蚂蚁淘代理EZ Biosystems™在中国的业务。试剂EZ Biosystems™代理北京，试剂EZ Biosystems™代理，EZ Biosystems™华东代理，EZ Biosystems™华北代理，EZ Biosystems™华中代理，EZ Biosystems™华南代理