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Sensitivity:Detectionofproteinaslowas25ng(BSA)canbeachievedin10min.Sensitivitycanbeextendedto10ngforsomeproteins(suchasBSA)withadditionalstainingtreatmentandwashingsteps.

Applications:DetectionofproteinsinnativeandSDS-PAGEgels.

Storage

Roomtemperatureinalightproofbottle.

Warning:Theinstructionsdescribedbelowinvolvehandingof“hot”liquid.Followthedirectionswithcautiontopreventburn.

1)Placeastandardminigelinamicrowavableplasticboxwith250mlofdeionizedwater(orsufficientamount)tocoverthegel.Looselycovertheboxandmicrowavethegelfor3min.(highsetting).

2)Discardthewater.Repeatstep1.

3)Optionalstep:Formaximumdetectionsensitivity,repeatstep1twomoretimes(toatotalof4waterwashings).

4)Submergethegelin50ml(orsufficientamount)ofBlueFastsolution.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Formaximumdetection,submergethegelwith100mlofBlueFastsolution,andmicrowavethegelfor4min.

5)Discardstain.Add250mlofdeionizedwater.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Discardwash.Add250mlofdeionizedwater.

6)Optionalstep:Formaximumdetectionsensitivity,repeatstep5.

7)Optionalstep:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Toremoveitcompletely,washthegel3times,30min.eachwithrocking,with250mlofdeionizedwater,orsimplyleavethegelinwaterovernight.

Q:WhatisBlueFast?

A:BlueFastisaproprietaryformulationofcoomassieblueG-250stainsolution.Itsuniqueformulaworksitsmagicwithheattospeeduptheprocessofproteinstainingwithhighdetectionsensitivity.

Q:WhatisthesensitivityofBlueFast?

A:BlueFastworksbestfordetectionofproteins15kDaandabove.It’sdetectionsensitivityvariesfromproteintoproteindependingontheircomposition.Ingeneral,BlueFastdetectsaslowas25ngofBSAinnativeandSDS-PAGEwithin10minutesusingthestandardprocedure.ItispossIBLetoincreasethesensitivityofBlueFastdetectiontoaslowas10ngofBSA.Toachievethis,repeatthewatertreatmentsteptwomoretimes(toatotalof4treatments),increasetheBlueFaststainingstepfrom2to4minutes,andrepeatthepoststainingwashwithheatingonemoretime.

Q:HowdoesthedetectionsensitivityofBlueFastcomparedtotrADItionalcoomassiebluestainingmethodandsilverstainingmethod?

A:BlueFastisbasedoncolloidalcoomassiebluestainingtechnologysoitismoresensitivethanthetraditionalcoomassiebluestainingmethod.SilverstainingprovideshigherdetectionsensitivityforsomeproteinsthanBlueFast.However,silverstainingismoredifficulttoworkwithanditshighdetectionsensitivityisnotuniversallyapplicabletoallproteins.

Q:Howdoesthewatertreatmentwork?

A:BoilingofthegelinwaterpriortotheBlueFaststepremovessubstancesthatcaninhibittheinteractionbetweenBlueFastäandproteinsinthegel.

Q:Whatistheeffectofboilingonpolyacrylamidegel?

A:Boilingdoesnotdestroythepolyacrylamidegel.Theonlybeneficialeffectofboilingonthepolyacrylamidegelisitfixesthegelsoitwillnotexpandinwater.

Q:WhyproteinsdetectedbyBlueFastshowalightershadeofbluecomparedtothecolorseenwhenusingthetraditionalcoomassiebluestainingmethod?

A:TheuniqueformulationofBlueFastäyieldsalighterblue-coloredstainedproteinthanthatproducedbythetraditionalcoomassiebluestainingmethod.Thisdoesnotdecreasethesensitivityofproteindetection.

Q:CanBlueFastbereused?

A:Yes,butwedon’trecommenditunlessoneisworkingwithabundantamountofproteins.Detectionsensitivitydecreases50%whenBlueFastisusedthesecondtime.Therefore,unlessthereisahighconcentrationofproteininthegel,wedonotrecommendusingBlueFastasecondtime.

Q:CanproteinsbetransferredtoamembranefromagelaftertheyhavebeenstainedbyBlueFast?

A:Noformostproteins.OnceproteinsarestainedbyBlueFast,theycannotbetransferred.ItismisleadingtoseebluebandsonamembraneifoneattemptstotransferBlueFaststainedproteinsfromgel.Thetransferredbluebandsarecoomassiebluedyes.SinceproteindetectionbyBlueFastissorapid,werecommendrunningtwoidenticalgelssimultaneously.Leaveonegelintheelectrophoresisapparatus,stainthesecondgelwithBlueFastandobtaintheresultswithin10minutes.Basedontheresultsofthestainedgel,theuserthencandecidewhethertotransfertheunstainedgeltoamembrane.

Q:CanIremovethelight-bluebackgroundproducedbyBlueFastcompletely?

A:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Tocompletelyremovethelight-bluebackground,onecanrepeatthepoststainingwashstepwithheatingseveraltimes,leavethegelin500mlofwaterandchangethewaterhourly,orsimplyleavethegelinwaterovernight.

Q:CanItreattheBlueFaststainedgelwithgeldryingreagentscontainingglycerolandethanol?

A:Yes.

NOTE:Werecommendwashingthegelstwice(10minuteseach)withwaterandthenprocessingthegelwithgeldryingreagents.

Q:CanIsequenceproteinsstainedbyBlueFastusingmassspec?

A:Yes.Excisetheproteinofinterestedfromthestainedgel.Washtheexcisedgel3timeswithwater(onehoureach)orleaveitinwaterovernight.Thenproceedtothesequencingprocedure.

MATERIALSAFETYDATASHEET

TRADENAME:BlueFastProteinStainingSolution

DATEOFISSUE:February8,2017

SKU#:1900011002(100ml),1900010013(1000ml)

TRADENAME:BlueFastProteinStainingSolution

Manufacturer:EtonBioscienceInc.,5820OberlinDrive,Suite108,SanDiego,CA92121

InformationTelephone:800.758.1630

EmergencyTelephone:800.758.1630

SECTIONII:COMPOSITION/INFORMATIONONINGREDIENTS

CHEMICALCHARACTERIZATION:

	CAS#
TritonX-100	9002-93-1
BrilliantBlueG250	6104-58-1
HydrochloricAcid	7467-01-0
Ethanol	64-17-5

HAZARDOUSCOMPONENTS:Theproductcontainsnosubstanceswhichattheirgivenconcentration,areconsideredtobehazardoustohealth.

SECTIONIII:HAZARDIDENTIFICATION

Irritatingtoeyesandskin.Maycausesensitizationbyskincontact.

SECTIONIV:FIRSTAIDMEASURES

GeneralInformation:Removeandwashcontaminatedclothingpromptly.

INCASEOFINHALATION:Supplyfreshairandthenconsultadoctor.

INCASEOFEYECONTACT:Immediatelyflusheyeswithcopiousamountsofwaterfor15minutes.

INCASEOFSKINCONTACT:Immediatelywashskinwithsoapandcopiousamountsofwaterandconsultadoctor.Removecontaminatedclothingandshoes.Washclothingbeforereuse.Cleanshoesthoroughlybeforereuse.

IFSWALLOWED:Rinsemouthwithcopiousamountsofwater.Donotinducevomiting.Nevergiveanythingbymouthtoaunconsciousperson.Thenconsultadoctor.

SECTIONV:FIREFIGHTINGMEASURES

EXTINGUISHINGAGENTS:

Suitable:UseDrychemical,waterspray,carbondioxideorfoam.

SECTIONVI:ACCIDENTALRELEASEMEASURES

PRECAUTIONARYMEASURES:Nospecialmeasuresrequired.

CLEAN-UPPROCEDURES:Nospecialmeasuresrequired.

SECTIONVII:HANDLINGANDSTORAGE

HANDLING:Avoidinhalationofvapourormist.Keepawayfrosourcesofignition.Nosmoking.

STORAGE:Keepcontainertightlyclosedinadryandwell-ventilatedplace.

SECTIONVIII:EXPOSURECONTROLS/PERSONALPROTECTION

Theroutineprecautionarymeasuresforhandlingchemicalsshouldbefollowedsuchaswearingchemicalsafetygoggles,rubbergloves,rubberboots,NIOSH/MSHA-Approvedrespiratorwhenhandlingchemicalsandwashthoroughlyafterhandling.Handlechemicalswithcareandavoidcontactwitheyes,skin,oronclothing.Usesafetyshowerandeyebathasfirstaidmeasuresifexposedtochemicals.

SECTIONIX:PHYSICALANDCHEMICALPROPERTIES

PHYSICALSTATE/FORM:Liquid

TRADENAME:BluefastProteinStainingSolution

COLOR:Blue

ODOR:None

pHFACTOR:(20°C)2.5-3.5

VISCOSITY:(20°C)N/A

MELTINGPOINT:N/A

BOILINGPOINT:N/A

IGNITIONTEMPERATURE:N/A

FLASHPOINT:N/A

EXPLOSIONLEVEL:LOWER:N/A

UPPER:N/A

VAPORPRESSURE:(20°C)N/A

SPECIFICGRAVITY:(20°C)N/A

SOLUBILITYINWATER:(20°C)Soluble

SECTIONX:STABILITYANDREACTIVITY

SUBSTANCESTOBEAVOIDED:Oxidizingagents.

DANGEROUSREACTIONS:Nodangerousreactionsknown.

HAZARDOUS,COMBUSTION,ORDECOMPOSITIONPRODUCTS:

Undernormalconditionsofstorageandhandling,hazardous,combustionordecompositionproductsshouldnotbeproduced.

SECTIONXI:TOXICOLOGICALINFORMATION

TOXICITYDATA:N/A

ACUTEEFFECTSINHALATION:N/A.

EYECONTACT:Maycauseeyeirritation.

SKINCONTACT:Maycauseskinirritation.

INGESTION:Maybeharmfulifswallowed.

PROLONGEDEXPOSURE:N/A

CHRONICEFFECTS:N/A

RTECSnumber:N/A

ADDITIONALINFORMATION:

Theproductshouldbehandledwithroutineprecautionarymeasuresforhandlingchemicals.Additionalharmfulpropertiescannotberuledout.

SECTIONXII:ECOLOGICALINFORMATION

Noinformationavailable.

SECTIONXIII:DISPOSALCONSIDERATIONS

Chemicalwastegeneratorsmustdeterminewhetheradiscardedchemicalisclassifiedasahazardouswaste.USEPAguidelinesfortheclassificationdeterminationarelistedin40CFRParts261.3.Additionally,wastegeneratorsmustconsultstateandlocalhazardouswasteregulationstoensurecompleteandaccurateclassification.

SECTIONXIV:TRANSPORTINFORMATION

DOTREGULATIONS:

UNNumber:1170Class:3Packinggroup:III

ProperShippingName:EthanolSolutions

SECTIONXV:REGULATORYINFORMATION

Noinformationavailable.

SECTIONXVI:OTHERINFORMATION

Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation,andweassumenoliabilityresultingfromitsuse.Usersshouldmaketheirowninvestigationstodeterminethesuitabilityoftheinformationfortheirparticularpurposes.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.

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