SKU#: 2101241001

Lot No. 03C01

Ig type: rabbit IgG

Size: 100μg/vial

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Immunogen: A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids.

Purity: Immunogen affinity purified.

Applications:

Western blot: At 1-2μg/ml with the appropriate system to detect Annexin I in cells and tissues.

Immunohistochemistry (P): At 1-2μg/ml to detect Annexin I in formalin fixed and paraffin embedded tissues. Digesting the sections is required.

Immunocytochemistry: Suitable

Other applications have not been tested.

Optimal dilutions should be determined by end user.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg, Thimerosal, 0.05mg NaN3.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500μg/ml.

1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow,

E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human

lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81,

1986. .

2. Kovacic RT, Tizard R, Cate RL, FreyAZ, Wallner

BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep

17;30(37):9015-21.

3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I

regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.