- 转铁蛋白和apotranferrins
- Lab Reagents: Smart Buffers and Reagents
- 分子生物学用酶：分子生物学用酶
- 蛋白质纯化：阿波罗20毫升超滤
- 免疫球蛋白
- qPCR：基因分析和基因面板
- PCR : PCR Products
- 牛奶和血小板蛋白
- DNA & RNA Sequencing : Dye Terminator clean-up
- Aptamers: Catalogue Aptamers
- Peptide Synthesis : Amino Acids
- 运输和其他蛋白质
- DNA: Whole DNA
- STEM学习：STEM工具包
- 低内毒素水平蛋白质
- 提取和纯化-RNA：组织/培养
- 转染-EZ生物系统：细胞系
- 凝血因子
- 分析试剂盒
- 抗体
- Gel Electrophoresis: Gel Electrophoresis
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- α1酸性糖蛋白(a1AGP)重组蛋白品牌:古朵生物国产/进口
Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1028-02 | 8-oxo-dG-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1028-90 | 8-oxo-dG-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1028-95 | 8-oxo-dG-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
| 8-oxo-dG-CE Phosphoramidite |
Catalog Number: 10-1028-xx
Description: 8-oxo-dG-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C44H54N7O9P | M.W.: 855.93 | F.W.: 345.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 24 hours DNA Damage/RepairCellular DNA is constantly being damaged by oxidation andalkylation, by free radicals, and by ultraviolet and ionizing radiation. Thebody has therefore evolved a number of repair enzyme systems to excise andrepair these lesions. The 8-oxo purine monomers allow investigation of thestructure and activity of oligonucleotides containing an 8-oxo mutation which isformed naturally when DNA is subjected to oxidative conditions or ionizingradiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that arestructural components of alanine transfer RNA. Dihydrouracil and the hydroxypyrimidines are major base damage products formed by exposure of DNA to ionizingradiation. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
MSDS
Glen Report 7.1: NEW MINOR BASES
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
Synthetic oligonucleotides containing 8-oxo-dG must be cleaved and deprotected with ammonium hydroxide containing 0.25M 2-mercaptoethanol to avoid oxidative degradation of 8-oxo-dG sites.
Frequently Asked Technical Question
QUESTION: How do you deprotect 8-oxo-dG?
RESPONSE:Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1028 | 8-oxo-dG | 294 | 5.2 | 250 | 6.7 | 5.9 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 2.92 | 84 | 50.4 | 31.5 | 22.91 | 16.8 | 4.2 |
| 10-1028-90 | 100µmoles | .086grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1028-95 | 50µmoles | .043grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .07 | 80.8 | 50.5 | 36.73 | 5.05 | |
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1028-95 | 50µmoles | .043grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .07 | 82.4 | 51.5 | 37.45 | ||
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1028-95 | 50µmoles | .043grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
