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蚂蚁淘在线 / 品牌中心 / Athens Research / 雅典研究院/坎比奥-卓越分子生物学/0.5ml/EZT-AR42-1-05

雅典研究院/坎比奥-卓越分子生物学/0.5ml/EZT-AR42-1-05

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货号：EZT-AR42-1-05
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品牌：Athens Research
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Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)

AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-AR42-1-05AR42J Cell Avalanche™ Transfection Reagent0.5ml£399.00QuantityAdd to Order
EZT-AR42-1-15AR42J Cell Avalanche™ Transfection Reagent1.5ml£699.00QuantityAdd to Order

Please find below the related products

  • ViewAvalanche-in vivo Transfection Reagent
  • ViewAvalanche-Omni Transfection Reagent

AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)

AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)

EZ Biosystems

Cell Line Information:

Designations: AR42J Organism: Rattus norvegicus (rat) Strain: Wistar Tissue: pancreas; exocrine; tumor Morphology: epithelial Growth properties: adherent Tumorigenic: yes, in athymic mice Products: amylase and other exocrine enzymes Receptors expressed: insulin; glucocorticoid Depositors: N.W. Jessop The cells grow slowly, in clusters. They tend to pile up and appear refractile. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.

The Transfection Reagent:

AR42J Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on AR42J Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for AR42J Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of AR42J Cell Avalanche™ Transfection Reagent for transfection on AR42J Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into AR42J Cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AR42J cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AR42J cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AR42J Cell Avalanche Transfection Reagent.
For Other Cells:
AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell) can also be used on the following cells with high transfection efficiencies.

Capan-1 Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell 293 Cell 293T/17 Cell SK-OV-3 Cell U-2 OS cell Saos-2 Cell SK-N-SH Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Research Use Only. Not for use in diagnostic or therapeutic procedures.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Athens Research & Technology 公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。 我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，Athens Research & Technology 公司能够根据您的项目要求提供无与伦比的试剂。